focal adhesion kinase fak (d5o7u) xp® rabbit mab antibody Search Results


fak  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc fak
TG2–FN clusters <t>activate</t> <t>β-catenin</t> in OC cells. A , WB for <t>p-FAK</t> Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in A and B . C , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). D , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗∗∗ p < 0.0001). All Abs were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in C and D . E , IF staining for TG2 (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN for 2 h. F and G , quantification of Alexa Fluor 488 ( green ) and Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). H , OVCAR-5 cells sh-Ctr or sh-TG2 KD were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗ p < 0.001). I , real-time PCR for c-Myc in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN-coated plates for 2 h (N = 6; ∗∗∗ p < 0.001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; KD, knockdown; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.
Fak, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fak/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
fak - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier
focal adhesion kinase fak (d5o7u) xp ® rabbit mab antibody  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc focal adhesion kinase fak (d5o7u) xp ® rabbit mab antibody
TG2–FN clusters <t>activate</t> <t>β-catenin</t> in OC cells. A , WB for <t>p-FAK</t> Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in A and B . C , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). D , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗∗∗ p < 0.0001). All Abs were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in C and D . E , IF staining for TG2 (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN for 2 h. F and G , quantification of Alexa Fluor 488 ( green ) and Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). H , OVCAR-5 cells sh-Ctr or sh-TG2 KD were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗ p < 0.001). I , real-time PCR for c-Myc in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN-coated plates for 2 h (N = 6; ∗∗∗ p < 0.001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; KD, knockdown; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.
Focal Adhesion Kinase Fak (D5o7u) Xp ® Rabbit Mab Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/focal adhesion kinase fak (d5o7u) xp ® rabbit mab antibody/product/Cell Signaling Technology Inc
Average 90 stars, based on 1 article reviews
focal adhesion kinase fak (d5o7u) xp ® rabbit mab antibody - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
junctional adhesion molecule jam-a (e6z7e) rabbit mab antibody  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc junctional adhesion molecule jam-a (e6z7e) rabbit mab antibody
TG2–FN clusters <t>activate</t> <t>β-catenin</t> in OC cells. A , WB for <t>p-FAK</t> Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in A and B . C , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). D , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗∗∗ p < 0.0001). All Abs were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in C and D . E , IF staining for TG2 (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN for 2 h. F and G , quantification of Alexa Fluor 488 ( green ) and Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). H , OVCAR-5 cells sh-Ctr or sh-TG2 KD were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗ p < 0.001). I , real-time PCR for c-Myc in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN-coated plates for 2 h (N = 6; ∗∗∗ p < 0.001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; KD, knockdown; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.
Junctional Adhesion Molecule Jam A (E6z7e) Rabbit Mab Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/junctional adhesion molecule jam-a (e6z7e) rabbit mab antibody/product/Cell Signaling Technology Inc
Average 90 stars, based on 1 article reviews
junctional adhesion molecule jam-a (e6z7e) rabbit mab antibody - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
a fak  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc a fak
TG2–FN clusters <t>activate</t> <t>β-catenin</t> in OC cells. A , WB for <t>p-FAK</t> Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in A and B . C , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). D , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗∗∗ p < 0.0001). All Abs were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in C and D . E , IF staining for TG2 (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN for 2 h. F and G , quantification of Alexa Fluor 488 ( green ) and Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). H , OVCAR-5 cells sh-Ctr or sh-TG2 KD were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗ p < 0.001). I , real-time PCR for c-Myc in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN-coated plates for 2 h (N = 6; ∗∗∗ p < 0.001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; KD, knockdown; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.
A Fak, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/a fak/product/Cell Signaling Technology Inc
Average 97 stars, based on 1 article reviews
a fak - by Bioz Stars, 2026-03
97/100 stars
  Buy from Supplier
antibodies against erbb3 d22c5  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc antibodies against erbb3 d22c5
TG2–FN clusters <t>activate</t> <t>β-catenin</t> in OC cells. A , WB for <t>p-FAK</t> Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in A and B . C , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). D , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗∗∗ p < 0.0001). All Abs were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in C and D . E , IF staining for TG2 (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN for 2 h. F and G , quantification of Alexa Fluor 488 ( green ) and Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). H , OVCAR-5 cells sh-Ctr or sh-TG2 KD were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗ p < 0.001). I , real-time PCR for c-Myc in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN-coated plates for 2 h (N = 6; ∗∗∗ p < 0.001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; KD, knockdown; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.
Antibodies Against Erbb3 D22c5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies against erbb3 d22c5/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
antibodies against erbb3 d22c5 - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
p fak y397 d20b1  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc p fak y397 d20b1
TG2–FN clusters <t>activate</t> <t>β-catenin</t> in OC cells. A , WB for <t>p-FAK</t> Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in A and B . C , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). D , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗∗∗ p < 0.0001). All Abs were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in C and D . E , IF staining for TG2 (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN for 2 h. F and G , quantification of Alexa Fluor 488 ( green ) and Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). H , OVCAR-5 cells sh-Ctr or sh-TG2 KD were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗ p < 0.001). I , real-time PCR for c-Myc in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN-coated plates for 2 h (N = 6; ∗∗∗ p < 0.001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; KD, knockdown; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.
P Fak Y397 D20b1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p fak y397 d20b1/product/Cell Signaling Technology Inc
Average 96 stars, based on 1 article reviews
p fak y397 d20b1 - by Bioz Stars, 2026-03
96/100 stars
  Buy from Supplier
pan akt c67e7  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc pan akt c67e7
TG2–FN clusters <t>activate</t> <t>β-catenin</t> in OC cells. A , WB for <t>p-FAK</t> Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in A and B . C , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). D , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗∗∗ p < 0.0001). All Abs were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in C and D . E , IF staining for TG2 (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN for 2 h. F and G , quantification of Alexa Fluor 488 ( green ) and Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). H , OVCAR-5 cells sh-Ctr or sh-TG2 KD were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗ p < 0.001). I , real-time PCR for c-Myc in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN-coated plates for 2 h (N = 6; ∗∗∗ p < 0.001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; KD, knockdown; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.
Pan Akt C67e7, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pan akt c67e7/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
pan akt c67e7 - by Bioz Stars, 2026-03
95/100 stars
  Buy from Supplier
p erbb3 y1289  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc p erbb3 y1289
TG2–FN clusters <t>activate</t> <t>β-catenin</t> in OC cells. A , WB for <t>p-FAK</t> Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in A and B . C , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). D , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗∗∗ p < 0.0001). All Abs were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in C and D . E , IF staining for TG2 (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN for 2 h. F and G , quantification of Alexa Fluor 488 ( green ) and Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). H , OVCAR-5 cells sh-Ctr or sh-TG2 KD were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗ p < 0.001). I , real-time PCR for c-Myc in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN-coated plates for 2 h (N = 6; ∗∗∗ p < 0.001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; KD, knockdown; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.
P Erbb3 Y1289, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
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95/100 stars
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parp  (Cell Signaling Technology Inc)
99
Cell Signaling Technology Inc parp
TG2–FN clusters <t>activate</t> <t>β-catenin</t> in OC cells. A , WB for <t>p-FAK</t> Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in A and B . C , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). D , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗∗∗ p < 0.0001). All Abs were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in C and D . E , IF staining for TG2 (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN for 2 h. F and G , quantification of Alexa Fluor 488 ( green ) and Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). H , OVCAR-5 cells sh-Ctr or sh-TG2 KD were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗ p < 0.001). I , real-time PCR for c-Myc in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN-coated plates for 2 h (N = 6; ∗∗∗ p < 0.001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; KD, knockdown; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.
Parp, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti zeb1  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc anti zeb1
TG2–FN clusters <t>activate</t> <t>β-catenin</t> in OC cells. A , WB for <t>p-FAK</t> Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in A and B . C , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). D , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗∗∗ p < 0.0001). All Abs were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in C and D . E , IF staining for TG2 (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN for 2 h. F and G , quantification of Alexa Fluor 488 ( green ) and Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). H , OVCAR-5 cells sh-Ctr or sh-TG2 KD were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗ p < 0.001). I , real-time PCR for c-Myc in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN-coated plates for 2 h (N = 6; ∗∗∗ p < 0.001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; KD, knockdown; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.
Anti Zeb1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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thr41  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc thr41
TG2–FN clusters <t>activate</t> <t>β-catenin</t> in OC cells. A , WB for <t>p-FAK</t> Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in A and B . C , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). D , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗∗∗ p < 0.0001). All Abs were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in C and D . E , IF staining for TG2 (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN for 2 h. F and G , quantification of Alexa Fluor 488 ( green ) and Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). H , OVCAR-5 cells sh-Ctr or sh-TG2 KD were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗ p < 0.001). I , real-time PCR for c-Myc in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN-coated plates for 2 h (N = 6; ∗∗∗ p < 0.001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; KD, knockdown; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.
Thr41, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/thr41/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
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TG2–FN clusters activate β-catenin in OC cells. A , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in A and B . C , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). D , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗∗∗ p < 0.0001). All Abs were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in C and D . E , IF staining for TG2 (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN for 2 h. F and G , quantification of Alexa Fluor 488 ( green ) and Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). H , OVCAR-5 cells sh-Ctr or sh-TG2 KD were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗ p < 0.001). I , real-time PCR for c-Myc in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN-coated plates for 2 h (N = 6; ∗∗∗ p < 0.001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; KD, knockdown; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.

Journal: The Journal of Biological Chemistry

Article Title: Tissue transglutaminase activates integrin-linked kinase and β-catenin in ovarian cancer

doi: 10.1016/j.jbc.2022.102242

Figure Lengend Snippet: TG2–FN clusters activate β-catenin in OC cells. A , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, TG2, and GAPDH in OVCAR-5 cells stably transduced with scrambled- or TG2-targeting shRNA and plated on FN-coated plates for 30 min, 1 h, and 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in A and B . C , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, TG2, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios and TG2 expression levels (N = 3; ∗∗ p < 0.01, ∗∗∗ p < 0.01, and ∗∗∗∗ p < 0.0001). D , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells stably transduced with TG2-targeting shRNA, transfected with pCMV3- TGM2 -C-His, and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗ p < 0.05, ∗∗∗∗ p < 0.0001). All Abs were probed on the same membrane. For consistency, the same TG2 and GAPDH representative WB images and TG2 quantification are shown in C and D . E , IF staining for TG2 (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN for 2 h. F and G , quantification of Alexa Fluor 488 ( green ) and Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). H , OVCAR-5 cells sh-Ctr or sh-TG2 KD were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗ p < 0.001). I , real-time PCR for c-Myc in OVCAR-5 cells sh-Ctr or sh-TG2 KD plated on FN-coated plates for 2 h (N = 6; ∗∗∗ p < 0.001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; KD, knockdown; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.

Article Snippet: Polyclonal ILK (clone 4G9), nonphospho (active) β-catenin at Ser33/37 and Thr41 (clone D13A1), p-FAK Tyr576/577 , FAK (clone D5O7U), GSK-3α/β, and p-GSK-3α/β Ser21/9 (clone 37F11) antibodies were from Cell Signaling Technology; and GAPDH from Biodesign International.

Techniques: Stable Transfection, Transduction, shRNA, Expressing, Membrane, Transfection, Staining, Software, Luciferase, Control, Plasmid Preparation, Activity Assay, Real-time Polymerase Chain Reaction, Immunofluorescence, Knockdown, Binding Assay, Western Blot

Functional TG2 and integrin β1 inhibition disrupts β-catenin signaling in OC cells. A , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, and GAPDH in OVCAR-5 cells treated with inhibitory antibodies (Abs) directed against the FN-binding domain of TG2 (clone 4G3) or integrin β1 (clone P5D2) and/or Wnt-3A and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios (N = 3; ∗ p < 0.05; ∗∗ p < 0.01). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells treated with 4G3 or P5D2 Abs and/or Wnt-3A and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001). C , IF staining for phalloidin (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells treated with 4G3 or P5D2 Abs and/or Wnt-3A plated on FN for 2 h. D , quantification of Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). E , OVCAR-5 cells were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid, treated with 4G3 or P5D2 Abs and/or Wnt-3A, and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗∗ p < 0.0001). F , real-time PCR for c-Myc in OVCAR-5 cells treated with 4G3 or P5D2 Abs and/or Wnt-3A and plated on FN-coated plates for 2 h (N = 6; ∗∗∗∗ p < 0.0001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.

Journal: The Journal of Biological Chemistry

Article Title: Tissue transglutaminase activates integrin-linked kinase and β-catenin in ovarian cancer

doi: 10.1016/j.jbc.2022.102242

Figure Lengend Snippet: Functional TG2 and integrin β1 inhibition disrupts β-catenin signaling in OC cells. A , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, and GAPDH in OVCAR-5 cells treated with inhibitory antibodies (Abs) directed against the FN-binding domain of TG2 (clone 4G3) or integrin β1 (clone P5D2) and/or Wnt-3A and plated on FN-coated plates for 2 h. Densitometry quantifies p-FAK Tyr576/577 /FAK and p-ILK Ser246 /ILK ratios (N = 3; ∗ p < 0.05; ∗∗ p < 0.01). B , WB for non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells treated with 4G3 or P5D2 Abs and/or Wnt-3A and plated on FN-coated plates for 2 h. Densitometry quantifies non–p-active β-catenin expression levels and p-GSKα/β Ser21/9 /GSKα/β ratio (N = 3; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001). C , IF staining for phalloidin (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells treated with 4G3 or P5D2 Abs and/or Wnt-3A plated on FN for 2 h. D , quantification of Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). E , OVCAR-5 cells were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid, treated with 4G3 or P5D2 Abs and/or Wnt-3A, and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗∗ p < 0.0001). F , real-time PCR for c-Myc in OVCAR-5 cells treated with 4G3 or P5D2 Abs and/or Wnt-3A and plated on FN-coated plates for 2 h (N = 6; ∗∗∗∗ p < 0.0001). FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; TG2, tissue transglutaminase; WB, Western blot.

Article Snippet: Polyclonal ILK (clone 4G9), nonphospho (active) β-catenin at Ser33/37 and Thr41 (clone D13A1), p-FAK Tyr576/577 , FAK (clone D5O7U), GSK-3α/β, and p-GSK-3α/β Ser21/9 (clone 37F11) antibodies were from Cell Signaling Technology; and GAPDH from Biodesign International.

Techniques: Functional Assay, Inhibition, Binding Assay, Expressing, Staining, Software, Luciferase, Control, Plasmid Preparation, Activity Assay, Real-time Polymerase Chain Reaction, Immunofluorescence, Western Blot

ILK inhibition blocks β-catenin signaling in OC cells. A and B , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells plated on FN-coated plates for 2 h and treated or not with cpd-22 and/or Wnt-3A. Densitometry quantifies non–p-active β-catenin expression levels and p-FAK Tyr576/577 /FAK, p-ILK Ser246 /ILK, and p-GSKα/β Ser21/9 /GSKα/β ratios (N = 3; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same GAPDH representative WB images are shown in A and B . C and D , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells sh-Ctr or sh-ILK plated on FN for 2 h and treated or not with Wnt-3A. Densitometry quantifies ILK and non–p-active β-catenin expression levels and p-FAK Tyr576/577 /FAK, p-ILK Ser246 /ILK, and p-GSKα/β Ser21/9 /GSKα/β ratios (N = 3; ∗∗ p < 0.01; ∗∗∗ p < 0.001; and ∗∗∗∗ p < 0.0001). E , IF staining for phalloidin (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells plated on FN-coated plates for 2 h and treated or not with cpd-22 and/or Wnt-3A. F , quantification of Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). G , OVCAR-5 cells were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid prior to treatment with cpd-22 or Wnt-3A and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗∗ p < 0.0001). H , real-time PCR for c-Myc in OVCAR-5 cells plated on FN-coated plates for 2 h and treated or not with cpd-22 and/or Wnt-3A (N = 6; ∗∗∗∗ p < 0.0001). I , OVCAR-5 cells sh-Ctr or sh-ILK were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid prior to treatment with cpd-22 or Wnt-3A and plated on FN for 2 h (N = 6; ∗∗∗ p < 0.01; ∗∗∗ p < 0.001). J , real-time PCR for c-Myc in OVCAR-5 cells stably transduced with scrambled- or ILK-targeting shRNA plated on FN-coated plates for 2 h and treated or not with Wnt-3A (N = 6; ∗∗∗ p < 0.01; ∗∗∗ p < 0.001). cpd-22, compound 22; FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; WB, Western blot.

Journal: The Journal of Biological Chemistry

Article Title: Tissue transglutaminase activates integrin-linked kinase and β-catenin in ovarian cancer

doi: 10.1016/j.jbc.2022.102242

Figure Lengend Snippet: ILK inhibition blocks β-catenin signaling in OC cells. A and B , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells plated on FN-coated plates for 2 h and treated or not with cpd-22 and/or Wnt-3A. Densitometry quantifies non–p-active β-catenin expression levels and p-FAK Tyr576/577 /FAK, p-ILK Ser246 /ILK, and p-GSKα/β Ser21/9 /GSKα/β ratios (N = 3; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001). All antibodies (Abs) were probed on the same membrane. For consistency, the same GAPDH representative WB images are shown in A and B . C and D , WB for p-FAK Tyr576/577 , FAK, p-ILK Ser246 , ILK, non–p-active β-catenin, p-GSKα/β Ser21/9 , GSKα/β, and GAPDH in OVCAR-5 cells sh-Ctr or sh-ILK plated on FN for 2 h and treated or not with Wnt-3A. Densitometry quantifies ILK and non–p-active β-catenin expression levels and p-FAK Tyr576/577 /FAK, p-ILK Ser246 /ILK, and p-GSKα/β Ser21/9 /GSKα/β ratios (N = 3; ∗∗ p < 0.01; ∗∗∗ p < 0.001; and ∗∗∗∗ p < 0.0001). E , IF staining for phalloidin (Alexa Fluor 488, green ) and non–p-active β-catenin (Alexa Fluor 568, red ) in OVCAR-5 cells plated on FN-coated plates for 2 h and treated or not with cpd-22 and/or Wnt-3A. F , quantification of Alexa Fluor 568 ( red ) proteins was calculated by using Metamorph software (N = 3; ∗∗∗∗ p < 0.0001). G , OVCAR-5 cells were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid prior to treatment with cpd-22 or Wnt-3A and plated on FN for 2 h. Luciferase signal relative to Renilla activity is expressed as fold increase (N = 6; ∗∗∗∗ p < 0.0001). H , real-time PCR for c-Myc in OVCAR-5 cells plated on FN-coated plates for 2 h and treated or not with cpd-22 and/or Wnt-3A (N = 6; ∗∗∗∗ p < 0.0001). I , OVCAR-5 cells sh-Ctr or sh-ILK were cotransfected with TCF/LEF1 luciferase reporter and Renilla control plasmid prior to treatment with cpd-22 or Wnt-3A and plated on FN for 2 h (N = 6; ∗∗∗ p < 0.01; ∗∗∗ p < 0.001). J , real-time PCR for c-Myc in OVCAR-5 cells stably transduced with scrambled- or ILK-targeting shRNA plated on FN-coated plates for 2 h and treated or not with Wnt-3A (N = 6; ∗∗∗ p < 0.01; ∗∗∗ p < 0.001). cpd-22, compound 22; FAK, focal adhesion kinase; FN, fibronectin; GSKα/β, glycogen synthase kinase-3α/β; IF, immunofluorescence; ILK, integrin-linked kinase; LEF1, lymphoid enhancer–binding factor 1; OC, ovarian cancer; TCF, T-cell factor; WB, Western blot.

Article Snippet: Polyclonal ILK (clone 4G9), nonphospho (active) β-catenin at Ser33/37 and Thr41 (clone D13A1), p-FAK Tyr576/577 , FAK (clone D5O7U), GSK-3α/β, and p-GSK-3α/β Ser21/9 (clone 37F11) antibodies were from Cell Signaling Technology; and GAPDH from Biodesign International.

Techniques: Inhibition, Expressing, Membrane, Staining, Software, Luciferase, Control, Plasmid Preparation, Activity Assay, Real-time Polymerase Chain Reaction, Stable Transfection, Transduction, shRNA, Immunofluorescence, Binding Assay, Western Blot